Metals are subject to internal subcellular compartmentalization, altering their bioavailability. Thus, subcellular metal assessments are crucial in biomonitoring efforts. Metal distribution in three subcellular fractions (insoluble - ISF, thermolabile - TLF and thermostable - TSF) were determined by ICP-MS in Steno bredanensis specimens from Southeastern Brazil. Associations between metals, metallothionein (MT) and reduced glutathione (GSH) were also investigated. Differential metal-detoxification mechanisms were observed. MT detoxification was mostly noted for As, Cd, and Pb, while Cu, Cr, Hg, Ni, Se and Ti displayed lower MT-associations. Fe, Zn and Se, on the other hand, were poorly associated to MT, and mostly present in the ISF, indicating low bioavailability. This is the first report on subcellular Sn and Ti distribution in cetaceans and the first in this species in Brazil. Potential protective roles of essential metals against toxic elements are postulated. This study indicates that important biochemical detoxification information is obtained through subcellular fraction analyses in marine mammals.
Monteiro F., Lemos L.S., Moura J.F., Rocha R.C.C., Moreira I., Di Beneditto A.P., Kehrig H.A., Bordon I.C.A.C., Siciliano S., Saint'Pierre T.D. and Hauser-Davis R.A. (2019) Subcellular metal distributions and metallothionein associations in rough-toothed dolphins (Steno bredanensis) from Southeastern Brazil. Marine Pollution Bulletin 146: 263-273. 10.1016/j.marpolbul.2019.06.038
@article{Monteiro2019,
Title = {Subcellular metal distributions and metallothionein associations in rough-toothed dolphins (Steno bredanensis) from Southeastern Brazil},
Author = {Monteiro, Fernanda and Lemos, Leila S. and Moura, Jailson Fulgencio and Rocha, Rafael Christian Chávez and Moreira, Isabel and Di Beneditto, Ana Paula and Kehrig, Helena A. and Bordon, Isabella C.A.C. and Siciliano, Salvatore and Saint'Pierre, Tatiana D. and Hauser-Davis, Rachel Ann},
Editor = {},
Journal = {Marine Pollution Bulletin},
Year = {2019},
Pages = {263-273},
Volume = {146},
Doi = {10.1016/j.marpolbul.2019.06.038},
Abstract = {Metals are subject to internal subcellular compartmentalization, altering their bioavailability. Thus, subcellular metal assessments are crucial in biomonitoring efforts. Metal distribution in three subcellular fractions (insoluble - ISF, thermolabile - TLF and thermostable - TSF) were determined by ICP-MS in Steno bredanensis specimens from Southeastern Brazil. Associations between metals, metallothionein (MT) and reduced glutathione (GSH) were also investigated. Differential metal-detoxification mechanisms were observed. MT detoxification was mostly noted for As, Cd, and Pb, while Cu, Cr, Hg, Ni, Se and Ti displayed lower MT-associations. Fe, Zn and Se, on the other hand, were poorly associated to MT, and mostly present in the ISF, indicating low bioavailability. This is the first report on subcellular Sn and Ti distribution in cetaceans and the first in this species in Brazil. Potential protective roles of essential metals against toxic elements are postulated. This study indicates that important biochemical detoxification information is obtained through subcellular fraction analyses in marine mammals.},
}
TY - JOUR
AU - Monteiro, Fernanda
AU - Lemos, Leila S.
AU - Moura, Jailson Fulgencio
AU - Rocha, Rafael Christian Chávez
AU - Moreira, Isabel
AU - Di Beneditto, Ana Paula
AU - Kehrig, Helena A.
AU - Bordon, Isabella C.A.C.
AU - Siciliano, Salvatore
AU - Saint'Pierre, Tatiana D.
AU - Hauser-Davis, Rachel Ann
TI - Subcellular metal distributions and metallothionein associations in rough-toothed dolphins (Steno bredanensis) from Southeastern Brazil
T2 - Marine Pollution Bulletin
PY - 2019
SP - 263-273
VL - 146
DO - 10.1016/j.marpolbul.2019.06.038
AB - Metals are subject to internal subcellular compartmentalization, altering their bioavailability. Thus, subcellular metal assessments are crucial in biomonitoring efforts. Metal distribution in three subcellular fractions (insoluble - ISF, thermolabile - TLF and thermostable - TSF) were determined by ICP-MS in Steno bredanensis specimens from Southeastern Brazil. Associations between metals, metallothionein (MT) and reduced glutathione (GSH) were also investigated. Differential metal-detoxification mechanisms were observed. MT detoxification was mostly noted for As, Cd, and Pb, while Cu, Cr, Hg, Ni, Se and Ti displayed lower MT-associations. Fe, Zn and Se, on the other hand, were poorly associated to MT, and mostly present in the ISF, indicating low bioavailability. This is the first report on subcellular Sn and Ti distribution in cetaceans and the first in this species in Brazil. Potential protective roles of essential metals against toxic elements are postulated. This study indicates that important biochemical detoxification information is obtained through subcellular fraction analyses in marine mammals.
ER -
