Cellulose is the most abundant polymer in the living world. Its enzymatic degradation is a crucial step in the decomposition of plant litter. Despite a wide variety of enzymatic mechanisms contributing to cellulose degradation, some general patterns driven by extracellular enzymes have emerged, allowing estimates of cellulolytic activities in environmental samples. The basic procedure described in this chapter is to incubate natural, crystalline cellulose fibres with an extract from environmental samples containing enzymes. The amount of glucose moieties being released is measured and attributed to cellulolysis. These measurements give an estimate of the potential for complete degradation of crystalline cellulose into glucose under optimal pH conditions.
Zimmer M. (2020) Cellulases. In: Bärlocher F., Gessner M.O. & Graca M.A.S. (eds.). Methods to study litter decomposition – a practicle guide (pp. 397-403). 10.1007/978-3-030-30515-4_42
@inbook{Zimmer2020,
Title = {Cellulases},
Author = {Zimmer, Martin},
Editor = {Bärlocher, F. and Gessner, M.O. and Graca, M.A.S.},
Year = {2020},
Pages = {397-403},
Doi = {10.1007/978-3-030-30515-4_42},
Isbn = {978-3-030-30514-7},
Booktitle = {Methods to study litter decomposition – a practicle guide},
Abstract = {Cellulose is the most abundant polymer in the living world. Its enzymatic degradation is a crucial step in the decomposition of plant litter. Despite a wide variety of enzymatic mechanisms contributing to cellulose degradation, some general patterns driven by extracellular enzymes have emerged, allowing estimates of cellulolytic activities in environmental samples. The basic procedure described in this chapter is to incubate natural, crystalline cellulose fibres with an extract from environmental samples containing enzymes. The amount of glucose moieties being released is measured and attributed to cellulolysis. These measurements give an estimate of the potential for complete degradation of crystalline cellulose into glucose under optimal pH conditions.},
}
TY - CHAP
AU - Zimmer, Martin
ED - Bärlocher, Felix
ED - Gessner, Mark O.
ED - Graca, Manuel A. S.
TI - Cellulases
PY - 2020
SP - 397-403
DO - 10.1007/978-3-030-30515-4_42
SN - 978-3-030-30514-7
BT - Methods to study litter decomposition – a practicle guide
AB - Cellulose is the most abundant polymer in the living world. Its enzymatic degradation is a crucial step in the decomposition of plant litter. Despite a wide variety of enzymatic mechanisms contributing to cellulose degradation, some general patterns driven by extracellular enzymes have emerged, allowing estimates of cellulolytic activities in environmental samples. The basic procedure described in this chapter is to incubate natural, crystalline cellulose fibres with an extract from environmental samples containing enzymes. The amount of glucose moieties being released is measured and attributed to cellulolysis. These measurements give an estimate of the potential for complete degradation of crystalline cellulose into glucose under optimal pH conditions.
ER -
