Functionalised ceramic spawning tiles with probiotic Pseudoalteromonas biofilms designed for clownfish aquaculture

@article{Wesseling2015,
  Title = {Functionalised ceramic spawning tiles with probiotic Pseudoalteromonas biofilms designed for clownfish aquaculture},
  Author = {Wesseling, Wiebke and Wittka, Sabine and Kroll, Stephen and Soltmann, Christian and Kegler, Pia and Kunzmann, Andreas and Riss, Hans Wolfgang and Lohmeyer, Michael},
  Editor = {},
  Year = {2015},
  Pages = {57-66},
  Volume = {446},
  Doi = {10.1016/j.aquaculture.2015.04.017},
  Abstract = {To prevent marine fish egg clutches of substrate spawners from bacterial and fungal infestation, functionalised ceramic spawning tiles with probiotic bacterial biofilms were designed for clownfish aquaculture in this study. Therefore, mechanically stable ceramic spawning tiles made of alumina were fabricated with convenient substrate properties for Pseudoalteromonas immobilisation, i.e., hydrophobic matrix and hydrophilic surface properties. An effective biofilm formation was achieved by equilibration of these ceramic tiles in marine liquid medium containing 1 wt% starch and subsequent inoculation with two different Pseudoalteromonas strains. Biofilm formation was confirmed qualitatively by scanning electron microscopy (SEM) and quantitatively by photometrical measurements according to Safranin O staining. Furthermore, antagonistic effects originating from Pseudoalteromonas biofilms against Paecilomyces lilacinus were detected by SEM, which are possibly due to bioactive molecules. Consequently, these innovative microbiologically conditioned ceramic spawning tiles are promising candidates to prevent fish egg clutches from pathogenic infestation, which leads to an improved aquaculture of substrate spawners such as clownfishes.},
}

TY  - JOUR
AU  - Wesseling, Wiebke
AU  - Wittka, Sabine
AU  - Kroll, Stephen
AU  - Soltmann, Christian
AU  - Kegler, Pia
AU  - Kunzmann, Andreas
AU  - Riss, Hans Wolfgang
AU  - Lohmeyer, Michael
TI  - Functionalised ceramic spawning tiles with probiotic Pseudoalteromonas biofilms designed for clownfish aquaculture
PY  - 2015
SP  - 57-66
VL  - 446
DO  - 10.1016/j.aquaculture.2015.04.017
AB  - To prevent marine fish egg clutches of substrate spawners from bacterial and fungal infestation, functionalised ceramic spawning tiles with probiotic bacterial biofilms were designed for clownfish aquaculture in this study. Therefore, mechanically stable ceramic spawning tiles made of alumina were fabricated with convenient substrate properties for Pseudoalteromonas immobilisation, i.e., hydrophobic matrix and hydrophilic surface properties. An effective biofilm formation was achieved by equilibration of these ceramic tiles in marine liquid medium containing 1 wt% starch and subsequent inoculation with two different Pseudoalteromonas strains. Biofilm formation was confirmed qualitatively by scanning electron microscopy (SEM) and quantitatively by photometrical measurements according to Safranin O staining. Furthermore, antagonistic effects originating from Pseudoalteromonas biofilms against Paecilomyces lilacinus were detected by SEM, which are possibly due to bioactive molecules. Consequently, these innovative microbiologically conditioned ceramic spawning tiles are promising candidates to prevent fish egg clutches from pathogenic infestation, which leads to an improved aquaculture of substrate spawners such as clownfishes.
ER  -