Vibrio cholerae in waters of the Sunderban mangrove: relationship with biogeochemical parameters and chitin in seston size fractions
Rubén J. Lara
Sucharit B. Neogi
Mohammad S. Islam
Zahid H. Mahmud
Shafiqul Islam
Debasish Paul
Biniam B. Demoz
Shinji Yamasaki
Gopinath B. Nair
Gerhard Kattner
Abstract
Wetland dynamics are probably linked to cholera endemicity in South Asia. We focus on links between Vibrio cholerae abundance, chitin content and suspended particle load in size fractions of suspended particulate matter (SPM) along the salinity gradient of Sunderban mangrove waters. SPM decreased downstream, while salinity increased from 0.2 to 4. Particulate organic carbon (90 ± 25 μM) and nitrogen (9.1 ± 3.3 μM) highly correlated with SPM and turbidity, suggesting a significant contribution of fine particles to organic matter. Total chitin ranged 1–2 mg/l and decreased downstream. The distribution among size fractions of SPM, chitin and V. cholerae O1 (the bacterial serogroup mainly associated with cholera epidemics) was similar, with ~98% of the total in the fraction <20 μm. In comparison, the number of V. cholerae O1 attached to zooplankton and microplankton size classes >20 μm was almost negligible, in contrast to usual assumptions. Thus, microdetritus, nanoplankton and fungal cells in size classes <20 μm represent a chitinaceous substrate on which V. cholerae can grow and survive. Total bacteria, cultivable vibrios and V. cholera O1 increased 5–10 times downstream, together with salinity and nitrite concentration. Overall, nitrate and silicate concentrations were relatively constant (>22 μM N and 100 μM Si). However, nitrite increased ~9 times in the outer sector, reaching ~1.2 μM N, probably as a result of increased abundance of nitrate-reducing vibrios. A characterization of Vibrio habitats that takes account of the presence of nitrate-reducing bacteria could improve the understanding of both mangrove nitrogen cycling and cholera seasonality.
Lara R.J., Neogi S.B., Islam M.S., Mahmud Z.H., Islam S., Paul D., Demoz B.B., Yamasaki S., Nair G.B. and Kattner G. (2011) Vibrio cholerae in waters of the Sunderban mangrove: relationship with biogeochemical parameters and chitin in seston size fractions. Wetlands Ecology and Management 19(1): 109-119. 10.1007/s11273-010-9204-0
@article{Lara2011,
Title = {Vibrio cholerae in waters of the Sunderban mangrove: relationship with biogeochemical parameters and chitin in seston size fractions},
Author = {Lara, Rubén J. and Neogi, Sucharit B. and Islam, Mohammad S. and Mahmud, Zahid H. and Islam, Shafiqul and Paul, Debasish and Demoz, Biniam B. and Yamasaki, Shinji and Nair, Gopinath B. and Kattner, Gerhard},
Editor = {},
Journal = {Wetlands Ecology and Management},
Year = {2011},
Pages = {109-119},
Volume = {19},
Doi = {10.1007/s11273-010-9204-0},
Abstract = {Wetland dynamics are probably linked to cholera endemicity in South Asia. We focus on links between Vibrio cholerae abundance, chitin content and suspended particle load in size fractions of suspended particulate matter (SPM) along the salinity gradient of Sunderban mangrove waters. SPM decreased downstream, while salinity increased from 0.2 to 4. Particulate organic carbon (90 ± 25 μM) and nitrogen (9.1 ± 3.3 μM) highly correlated with SPM and turbidity, suggesting a significant contribution of fine particles to organic matter. Total chitin ranged 1–2 mg/l and decreased downstream. The distribution among size fractions of SPM, chitin and V. cholerae O1 (the bacterial serogroup mainly associated with cholera epidemics) was similar, with ~98% of the total in the fraction <20 μm. In comparison, the number of V. cholerae O1 attached to zooplankton and microplankton size classes >20 μm was almost negligible, in contrast to usual assumptions. Thus, microdetritus, nanoplankton and fungal cells in size classes <20 μm represent a chitinaceous substrate on which V. cholerae can grow and survive. Total bacteria, cultivable vibrios and V. cholera O1 increased 5–10 times downstream, together with salinity and nitrite concentration. Overall, nitrate and silicate concentrations were relatively constant (>22 μM N and 100 μM Si). However, nitrite increased ~9 times in the outer sector, reaching ~1.2 μM N, probably as a result of increased abundance of nitrate-reducing vibrios. A characterization of Vibrio habitats that takes account of the presence of nitrate-reducing bacteria could improve the understanding of both mangrove nitrogen cycling and cholera seasonality.},
}
TY - JOUR
AU - Lara, Rubén J.
AU - Neogi, Sucharit B.
AU - Islam, Mohammad S.
AU - Mahmud, Zahid H.
AU - Islam, Shafiqul
AU - Paul, Debasish
AU - Demoz, Biniam B.
AU - Yamasaki, Shinji
AU - Nair, Gopinath B.
AU - Kattner, Gerhard
TI - Vibrio cholerae in waters of the Sunderban mangrove: relationship with biogeochemical parameters and chitin in seston size fractions
T2 - Wetlands Ecology and Management
PY - 2011
SP - 109-119
VL - 19
DO - 10.1007/s11273-010-9204-0
AB - Wetland dynamics are probably linked to cholera endemicity in South Asia. We focus on links between Vibrio cholerae abundance, chitin content and suspended particle load in size fractions of suspended particulate matter (SPM) along the salinity gradient of Sunderban mangrove waters. SPM decreased downstream, while salinity increased from 0.2 to 4. Particulate organic carbon (90 ± 25 μM) and nitrogen (9.1 ± 3.3 μM) highly correlated with SPM and turbidity, suggesting a significant contribution of fine particles to organic matter. Total chitin ranged 1–2 mg/l and decreased downstream. The distribution among size fractions of SPM, chitin and V. cholerae O1 (the bacterial serogroup mainly associated with cholera epidemics) was similar, with ~98% of the total in the fraction <20 μm. In comparison, the number of V. cholerae O1 attached to zooplankton and microplankton size classes >20 μm was almost negligible, in contrast to usual assumptions. Thus, microdetritus, nanoplankton and fungal cells in size classes <20 μm represent a chitinaceous substrate on which V. cholerae can grow and survive. Total bacteria, cultivable vibrios and V. cholera O1 increased 5–10 times downstream, together with salinity and nitrite concentration. Overall, nitrate and silicate concentrations were relatively constant (>22 μM N and 100 μM Si). However, nitrite increased ~9 times in the outer sector, reaching ~1.2 μM N, probably as a result of increased abundance of nitrate-reducing vibrios. A characterization of Vibrio habitats that takes account of the presence of nitrate-reducing bacteria could improve the understanding of both mangrove nitrogen cycling and cholera seasonality.
ER -
